The TBX5 Polyclonal Antibody (CAB15590) is a valuable tool for researchers studying the TBX5 protein, a transcription factor crucial for heart and limb development. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to TBX5, it allows for the detection and analysis of this important protein in various cell types, making it ideal for research in developmental biology and cardiovascular disease.TBX5 is a key player in the regulation of genes involved in heart and limb formation, making it an essential target for studies on congenital heart defects and developmental disorders.
By understanding the functions of TBX5, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new targets for therapeutic interventions.Overall, the TBX5 Polyclonal Antibody (CAB15590) serves as a valuable tool for researchers interested in unraveling the molecular mechanisms governing heart and limb development, offering new possibilities for advancing our understanding of developmental biology and potential therapeutic strategies for related diseases.
Product Name:
TBX5 Rabbit Polyclonal Antibody
SKU:
CAB15590
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human TBX5 (NP_852259.1).
Sequence:
MADA DEGF GLAH TPLE PDAK DLPC DSKP ESAL GAPS KSPS SPQA AFTQ QGME GIKV FLHE RELW LKFH EV
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
HOS; TBX5
Positive Sample:
Mouse heart,Rat heart
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
58kDa
Observed MW:
58kDa
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.
Purification Method:
Affinity purification
Gene ID:
6910
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TBX5 Rabbit pAb (CAB15590) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
