The TBC1D4 Polyclonal Antibody (CAB18216) is a valuable tool for researchers studying TBC1D4, a key regulator of glucose metabolism and insulin signaling pathways. This antibody, generated in rabbits, is highly specific to human samples and has been extensively validated for use in Western blotting applications. By binding to the TBC1D4 protein, this antibody allows for the detection and analysis of TBC1D4 expression in a variety of cell types, making it ideal for studies in diabetes, metabolism, and endocrinology.TBC1D4, also known as AS160, plays a crucial role in the translocation of glucose transporter proteins to the cell membrane in response to insulin signaling, thereby regulating glucose uptake and metabolism.
Dysregulation of TBC1D4 has been implicated in insulin resistance and metabolic disorders, making it a promising target for therapeutic interventions aimed at improving glucose homeostasis and treating diabetes.Overall, the TBC1D4 Polyclonal Antibody is a valuable tool for researchers seeking to unravel the molecular mechanisms underlying glucose metabolism and insulin signaling, with potential implications for the development of novel treatments for metabolic disorders.
Product Name:
TBC1D4 Rabbit Polyclonal Antibody
SKU:
CAB18216
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1059-1298 of human TBC1D4 (NP_055647.2).
This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
9882
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat skeletal muscle, using TBC1D4 Rabbit pAb (CAB18216) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
