The TBC1D25 Antibody (PAC062759) is a valuable tool for research involving TBC1D25, a protein involved in cellular processes such as endosomal trafficking and autophagy. This polyclonal antibody, generated in rabbits, exhibits high reactivity with human samples and is optimized for use in Western blot applications. By binding specifically to the TBC1D25 protein, this antibody enables accurate detection and analysis in various cell types, making it suitable for studies in cell biology and molecular biology.TBC1D25 is a key player in regulating cellular processes related to vesicle trafficking and autophagy, making it a potential target for investigating diseases such as neurodegenerative disorders and metabolic conditions.
Understanding the function of TBC1D25 is essential for uncovering its role in cellular homeostasis and disease pathogenesis, ultimately guiding the development of targeted therapies. With its specificity and reliability, the TBC1D25 Antibody is a valuable tool for researchers exploring the functions of TBC1D25 in various biological contexts.
Antibody Name:
TBC1D25 Antibody (PACO62759)
Antibody SKU:
PACO62759
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human TBC1 domain family member 25 protein (1-219AA)
IHC image of PACO62759 diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO62759 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Acts as a GTPase-activating protein specific for RAB33B. Involved in the regulation of autophagosome maturation, the process in which autophagosomes fuse with endosomes and lysosomes.
Synonyms:
TBC1 domain family member 25, TBC1D25, OATL1
UniProt Protein Function:
TBC1D25: Acts as a GTPase-activating protein specific for RAB33B. Involved in the regulation of autophagosome maturation, the process in which autophagosomes fuse with endosomes and lysosomes. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Vesicle; GAPs; GAPs, RabChromosomal Location of Human Ortholog: Xp11.23Cellular Component: autophagic vacuole; cytoplasmic vesicleMolecular Function: protein bindingBiological Process: autophagy
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein with a TBC domain and may function as a Rab GTPase activating protein. This gene was previously known as ornithine aminotransferase-like 1, but has no similarity to ornithine aminotransferase. [provided by RefSeq, Jul 2008]
