The TARDBP Polyclonal Antibody (CAB13404) is a valuable tool for researchers studying TAR DNA-binding protein (TARDBP), a multifunctional nuclear protein involved in various cellular processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.TARDBP, also known as TDP-43, plays a crucial role in RNA processing and regulation, making it a key player in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD).
By targeting TARDBP with this polyclonal antibody, researchers can detect and analyze the protein in different cell types, providing valuable insights into its function and potential implications in disease pathology.With its specificity and reliability, the TARDBP Polyclonal Antibody is an essential tool for studying TARDBP biology and its involvement in neurodegenerative disorders. Researchers in the fields of neuroscience, molecular biology, and neurology will find this antibody indispensable for their research endeavors.
Antibody Name:
Anti-TARDBP Antibody
Antibody SKU:
CAB13404
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IF
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human TARDBP (NP_031401.1).
Application:
WB IF
Recommended Dilution:
WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:
Human, Mouse, Rat
Positive Samples:
SW620, HeLa, A-549, HepG2, Mouse brain, Rat brain
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human TARDBP (NP_031401.1).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
NCBI Summary:
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]
Western blot analysis of extracts of various cell lines, using TARDBP antibody (CAB13404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunofluorescence analysis of NIH-3T3 cells using TARDBP Rabbit pAb (CAB13404) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
