The TARDBP Polyclonal Antibody (CAB1183) is specifically designed for research involving TAR DNA-binding protein 43 (TDP-43), a nuclear protein involved in RNA processing and regulation. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications such as Western blotting and immunofluorescence.TDP-43 is a key player in RNA metabolism and has been linked to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD).
By targeting TDP-43, researchers can further investigate its role in disease pathology and potentially identify novel therapeutic targets.With its specificity and sensitivity, the TARDBP Polyclonal Antibody (CAB1183) is an essential tool for studying the function and dysregulation of TDP-43 in various cellular and disease contexts. Its versatility and reliability make it an invaluable resource for researchers in neuroscience, molecular biology, and neurodegenerative disease research.
Product Name:
TDP-43/TARDB Rabbit Polyclonal Antibody
SKU:
CAB1183
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TDP-43/TARDBP (NP_031401.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsRIP,1:20 - 1:50
Synonyms:
ALS10; TDP-43; TDP-43/TARDBP
Positive Sample:
HeLa,Jurkat,SW620,HT-29,A-673,Es-2,Mouse spleen
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
45kDa
Observed MW:
42kDa
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
Purification Method:
Affinity purification
Gene ID:
23435
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using TDP-43/TARDB Rabbit pAb (CAB1183) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
