Anti-TARDBP Antibody (CAB0538)
- SKU:
- CAB0538
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
Anti-TARDBP Antibody (CAB0538)
The TDP-43 Polyclonal Antibody (CAB0538) is a valuable tool for researchers studying TAR DNA-binding protein 43 (TDP-43), a key player in neurodegenerative diseases such as ALS and frontotemporal dementia. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By targeting TDP-43, researchers can investigate its role in RNA processing, protein aggregation, and neuronal dysfunction, providing insights into the pathogenesis of neurodegenerative disorders.
TDP-43 is a DNA/RNA-binding protein that regulates gene expression and RNA metabolism in the nucleus and cytoplasm. Abnormal accumulation of TDP-43 in the cytoplasm is a hallmark of ALS and other neurodegenerative diseases, leading to cellular dysfunction and neuronal death. By studying TDP-43 with this antibody, researchers can unravel the mechanisms underlying disease pathology and identify potential therapeutic targets for these devastating conditions.
| Antibody Name: | Anti-TARDBP Antibody |
| Antibody SKU: | CAB0538 |
| Antibody Size: | 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human TARDBP |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, K-562, Raji, Mouse brain |
| Immunogen: | A synthetic peptide of human TARDBP |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 23435 |
| Uniprot: | Q13148 |
| Cellular Location: | Nucleus |
| Calculated MW: | 31kDa/44kDa |
| Observed MW: | 43kDa |
| Synonyms: | ALS10, TDP-43, TARDBP, TDP43 |
| Background: | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. |
| NCBI Summary: | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13148 |
| NCBI GenInfo Identifier: | 20140568 |
| NCBI Gene ID: | 23435 |
| NCBI Accession: | |
| UniProt Related Accession: | Q13148 |
| Molecular Weight: | 44 |
| NCBI Full Name: | TAR DNA-binding protein 43 |
| NCBI Synonym Full Names: | TAR DNA binding protein |
| NCBI Official Symbol: | TARDBPÂ Â |
| NCBI Official Synonym Symbols: | ALS10; TDP-43Â Â |
| NCBI Protein Information: | TAR DNA-binding protein 43 |
| UniProt Protein Name: | |
| UniProt Gene Name: |
 | Western blot analysis of extracts of various cell lines, using TARDBP antibody (CAB0538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat heart using TARDBP antibody (CAB0538) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using TARDBP antibody (CAB0538) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using TARDBP antibody (CAB0538) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using TARDBP antibody (CAB0538) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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