The Tapasin Polyclonal Antibody (CAB1968) is a versatile research tool designed for the study of tapasin, a key protein involved in the antigen presentation process. By specifically binding to tapasin, this antibody enables the detection and analysis of tapasin expression in various cell types, making it an excellent choice for immunology and cell biology research.Tapasin is a crucial component of the major histocompatibility complex (MHC) class I antigen presentation pathway, playing a vital role in assembling MHC class I molecules with antigenic peptides for presentation to T cells. Understanding the function of tapasin is essential for unraveling the mechanisms of immune recognition and response.
Research on tapasin is particularly relevant in the fields of immunology, infectious diseases, and vaccine development.This polyclonal antibody, produced by rabbits, is highly reactive with human samples and has been rigorously validated for use in techniques such as Western blotting. By utilizing the Tapasin Polyclonal Antibody (CAB1968), researchers can gain valuable insights into the role of tapasin in immune surveillance and immune evasion, paving the way for innovative therapeutic strategies in the treatment of infectious diseases and cancer.
Product Name:
TAPBP Rabbit Polyclonal Antibody
SKU:
CAB1968
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-260 of human TAPBP (NP_757345.2).
Endoplasmic reticulum membrane, Single-pass type I membrane protein.
Calculated MW:
48kDa
Observed MW:
48kDa
This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
6892
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using TAPBP Rabbit pAb (CAB1968) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
