The TAF9B Polyclonal Antibody (CAB15846) is a valuable tool for researchers studying TATA-box binding protein associated factor 9B (TAF9B), a key component of the TFIID basal transcription factor complex. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.TAF9B is essential for accurate transcription initiation and regulation of gene expression, making it a critical player in cellular processes such as DNA repair, cell cycle progression, and development. The TAF9B Polyclonal Antibody binds specifically to the TAF9B protein, enabling researchers to detect and analyze its expression in various cell types.
Research involving TAF9B is important for understanding the molecular mechanisms underlying transcriptional regulation, and its dysregulation has been implicated in various diseases including cancer and developmental disorders. By utilizing the TAF9B Polyclonal Antibody, researchers can gain valuable insights into the roles of TAF9B in cellular processes and potentially discover new therapeutic targets for a range of diseases.
Product Name:
TAF9B Rabbit Polyclonal Antibody
SKU:
CAB15846
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TAF9B (NP_057059.2).
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is similar to one of the small subunits of TFIID, TBP-associated factor 9, and is also a subunit of TFIID. TAF9 and TAF9b share some functions but also have distinct roles in the transcriptional regulatory process.
Purification Method:
Affinity purification
Gene ID:
51616
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using TAF9B Rabbit pAb (CAB15846) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
