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TAF9B Rabbit Polyclonal Antibody (CAB15846)

SKU:
CAB15846
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:TAF9B Rabbit Polyclonal Antibody
SKU:CAB15846
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TAF9B (NP_057059.2).
Sequence:ILDD AKIY SSHA KKPN VDAD DVRL AIQC RADQ SFTS PPPR DFLL DIAR QKNQ TPLP LIKP YAGP RLPP DRYC LTAP NYRL KSLI KKGP NQGR LVPR LSVG A
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:DN7; DN-7; TAF9L; TAFII31L; TFIID-31; TAF9B
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:28kDa
Observed MW:28kDa

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is similar to one of the small subunits of TFIID, TBP-associated factor 9, and is also a subunit of TFIID. TAF9 and TAF9b share some functions but also have distinct roles in the transcriptional regulatory process.

Purification Method:Affinity purification
Gene ID:51616
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using TAF9B Rabbit pAb (CAB15846) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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