The TAF9 Polyclonal Antibody (CAB2021) is a valuable tool for researchers studying TAF9, a crucial component of the TFIID complex involved in transcription initiation. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the TAF9 protein, it enables precise detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and gene regulation.TAF9 is essential for the transcription of protein-coding genes, playing a vital role in controlling gene expression and maintaining cellular function.
Its involvement in the TFIID complex highlights its importance in regulating transcriptional processes, making it a key target for research in areas such as gene expression, cell differentiation, and development. Understanding the functions of TAF9 is critical for unraveling the complexities of gene regulation and unlocking potential therapeutic targets for various diseases.
Product Name:
TAF9 Rabbit Polyclonal Antibody
SKU:
CAB2021
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human TAF9 (NP_003178.1).
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. In human, TAF9 and AK6 (GeneID: 102157402) are two distinct genes that share 5' exons. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6880
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using TAF9 antibody (CAB2021) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
