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TAF9 Rabbit Polyclonal Antibody (CAB2021)

SKU:
CAB2021
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-TAF9 Antibody CAB2021
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-TAF9 Antibody CAB2021
€139 - €419
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Description

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Product Name:TAF9 Rabbit Polyclonal Antibody
SKU:CAB2021
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human TAF9 (NP_003178.1).
Sequence:MESG KTAS PKSM PKDA QMMA QILK DMGI TEYE PRVI NQML EFAF RYVT TILD DAKI YSSH AKKA TVDA DDVR LAIQ CRAD QSFT SPPP RDFL LDIA RQRN QTPL PLIK PYSG PRLP PDRY CLTA PNYR LKSL QKKA STSA GRIT VPRL SVGS VTSR PSTP TLGT PTPQ TMSV
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:TAF2G; TAFII31; TAFII32; MGC:5067; TAFII-31; TAFII-32; TAFIID32; STAF31/32; TAF9
Positive Sample:BT-474,HeLa,K-562
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:29kDa
Observed MW:20kDa

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. In human, TAF9 and AK6 (GeneID: 102157402) are two distinct genes that share 5' exons. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:6880
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using TAF9 antibody (CAB2021) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.

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