The TAF8 Polyclonal Antibody (PACO53870) is a crucial tool for researchers studying TAF8, a key component of the transcription factor complex involved in gene expression regulation. This antibody, produced in rabbits, exhibits high specificity towards human samples and has been validated for use in Western blotting applications.TAF8, also known as TBP-associated factor 8, is essential for the assembly and function of the transcriptional machinery, playing a pivotal role in the regulation of gene transcription.
Its involvement in various cellular processes makes it a valuable target for research in fields such as molecular biology and genetics.By targeting the TAF8 protein, this antibody enables the precise detection and analysis of TAF8 in different cell types, allowing researchers to gain valuable insights into its functions and mechanisms of action. Its reliability and versatility make it an ideal tool for studies aiming to unravel the complexities of gene regulation and transcriptional control.
Antibody Name:
TAF8 Antibody (PACO53870)
Antibody SKU:
PACO53870
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:5000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human Transcription initiation factor TFIID subunit 8 protein (122-311AA)
Western Blot. Positive WB detected in: Rat kidney tissue, Mouse liver tissue. All lanes: TAF8 antibody at 2.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 35, 38 kDa. Observed band size: 38 kDa.
Background:
Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo (By similarity).
TBN: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo. Belongs to the TAF8 family. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 6p21.1Cellular Component: nucleoplasm; nucleus; perinuclear region of cytoplasm; transcription factor TFIID complexMolecular Function: protein bindingBiological Process: maintenance of protein localization in nucleus; positive regulation of transcription, DNA-dependent; regulation of fat cell differentiation; snRNA transcription from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:
This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
