The TAF6L Polyclonal Antibody (CAB14369) is a valuable tool for researchers studying the TAF6L protein, a key component of the TFIID transcription factor complex. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the TAF6L protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and gene regulation.
TAF6L is essential for the assembly and stability of the TFIID complex, which plays a crucial role in initiating transcription of protein-coding genes. Dysregulation of TAF6L has been implicated in various diseases, including cancer and developmental disorders, highlighting its importance in cellular processes. By studying the function and regulation of TAF6L, researchers can gain valuable insights into gene expression mechanisms and potential therapeutic targets for a range of conditions.
Product Name:
TAF6L Rabbit Polyclonal Antibody
SKU:
CAB14369
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TAF6L (NP_006464.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
PAF65A; TAF6L
Positive Sample:
HeLa,HT-29,Jurkat,Mouse thymus,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
68kDa
Observed MW:
70kDa
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is a component of the PCAF histone acetylase complex and structurally similar to one of the histone-like TAFs, TAF6. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation.
Purification Method:
Affinity purification
Gene ID:
10629
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TAF6L Rabbit pAb (CAB14369) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
