The TAF5L Polyclonal Antibody (PAC021702) is a vital tool for researchers studying TAF5L, a key component of the transcription factor IID (TFIID) complex essential for RNA polymerase II-mediated transcription initiation. This antibody, produced in rabbits, exhibits high sensitivity and specificity towards human samples, and has been validated for use in Western blot applications. By binding to the TAF5L protein, this antibody enables precise detection and analysis in a variety of cell types, making it an invaluable asset for investigations in transcription regulation and gene expression.TAF5L is a critical factor in the assembly and function of the TFIID complex, which plays a pivotal role in the initiation of transcription by RNA polymerase II.
Understanding the role of TAF5L is crucial for unraveling the molecular mechanisms underlying gene expression and is essential for research in fields such as developmental biology, cancer biology, and epigenetics. By targeting TAF5L with this specific antibody, researchers can gain insights into the complex processes governing transcriptional regulation, opening up new avenues for therapeutic interventions in various diseases and conditions.
Antibody Name:
TAF5L Antibody (PACO21702)
Antibody SKU:
PACO21702
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human TAF5L.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Struhl K., Cell 94:1-4(1998).
Clonality:
Polyclonal
Conjugate:
Non-conjugated
Western blot analysis of extracts from HT-29 cells and COS-7 cells, using TAF5L antibody.
TAF5L: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Belongs to the WD repeat TAF5 family. 2 isoforms of the human protein are produced by alternative splicing.
Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter
NCBI Summary:
The product of this gene belongs to the WD-repeat TAF5 family of proteins. This gene encodes a protein that is a component of the PCAF histone acetylase complex. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors to facilitate complex assembly and transcription initiation. The encoded protein is structurally similar to one of the histone-like TAFs, TAF5. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
