The TAF5 Polyclonal Antibody (CAB7221) is a valuable tool for researchers studying the TAF5 protein, a key component of the TFIID complex involved in transcriptional regulation. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the TAF5 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in molecular biology and gene expression research.TAF5, a subunit of the TFIID complex, plays a crucial role in the initiation of transcription by RNA polymerase II. Its involvement in regulating gene expression makes it a target of interest for studies on development, differentiation, and disease.
By understanding the functions of TAF5, researchers can gain insights into the mechanisms underlying various biological processes and potentially uncover new therapeutic strategies for conditions related to gene dysregulation.Overall, the TAF5 Polyclonal Antibody (CAB7221) offers a reliable and effective means of studying the TAF5 protein in human samples, providing valuable data for advancing our understanding of transcriptional regulation and its implications in various biological processes.
Product Name:
TAF5 Rabbit Polyclonal Antibody
SKU:
CAB7221
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 656-800 of human TAF5 (NP_008882.2).
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6877
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse brain, using TAF5 antibody (CAB7221) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
