TAF4B Antibody (PACO59317)
- SKU:
- PACO59317
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | TAF4B Antibody (PACO59317) |
| Antibody SKU: | PACO59317 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Transcription initiation factor TFIID subunit 4B protein (132-244AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO59317 diluted at 1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of A549 cells with PACO59317 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis. |
| Synonyms: | Transcription initiation factor TFIID subunit 4B (Transcription initiation factor TFIID 105 kDa subunit) (TAF(II)105) (TAFII-105) (TAFII105), TAF4B, TAF2C2 TAFII105 |
| UniProt Protein Function: | TAF4B: Cell type-specific subunit of TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAFII105 is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Belongs to the TAF4 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 18q11.2 Cellular Component: nucleolus; nucleoplasm Molecular Function:DNA binding; NF-kappaB binding Biological Process: RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter Disease: Spermatogenic Failure 13 |
| NCBI Summary: | TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] |
| UniProt Code: | Q92750 |
| NCBI GenInfo Identifier: | 119370533 |
| NCBI Gene ID: | 6875 |
| NCBI Accession: | Q92750.2 |
| UniProt Secondary Accession: | Q92750,Q29YA4, Q29YA5, |
| UniProt Related Accession: | Q92750 |
| Molecular Weight: | 63,364 Da |
| NCBI Full Name: | Transcription initiation factor TFIID subunit 4B |
| NCBI Synonym Full Names: | TATA-box binding protein associated factor 4b |
| NCBI Official Symbol: | TAF4BÂ Â |
| NCBI Official Synonym Symbols: | SPGF13; TAF2C2; TAFII105Â Â |
| NCBI Protein Information: | transcription initiation factor TFIID subunit 4B |
| UniProt Protein Name: | Transcription initiation factor TFIID subunit 4B |
| UniProt Synonym Protein Names: | Transcription initiation factor TFIID 105 kDa subunit; TAF(II)105; TAFII-105; TAFII105 |
| Protein Family: | Transcription initiation factor |
| UniProt Gene Name: | TAF4BÂ Â |
| UniProt Entry Name: | TAF4B_HUMAN |
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