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TAF4B Antibody (PACO59317)

SKU:
PACO59317
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
IHC
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • TAF4B Antibody PACO59317
  • TAF4B Antibody PACO59317
€359

Description

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TAF4B Antibody (PACO59317)

The TAF4B Monoclonal Antibody (PACO59317) is a valuable tool for researchers studying transcription regulation and gene expression. This antibody, developed against TAF4B, a key component of the transcription factor IID (TFIID) complex, is highly specific and sensitive for detecting TAF4B protein levels in human samples. Validated for use in immunofluorescence and immunohistochemistry applications, this antibody enables the visualization and analysis of TAF4B in various cell types and tissues.

TAF4B is essential for the assembly of the TFIID complex, which plays a critical role in the initiation of transcription by RNA polymerase II. Dysregulation of TAF4B expression has been linked to abnormal gene expression patterns and various disease states, including cancer. By studying the function and regulation of TAF4B, researchers can gain insights into the mechanisms underlying transcriptional control and potentially identify novel therapeutic targets for cancer and other diseases.

Antibody Name:TAF4B Antibody (PACO59317)
Antibody SKU:PACO59317
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, IHC, IF
Recommended Dilutions:ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200
Species Reactivity:Human
Immunogen:Recombinant Human Transcription initiation factor TFIID subunit 4B protein (132-244AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageIHC image of PACO59317 diluted at 1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product ImageImmunofluorescence staining of A549 cells with PACO59317 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis.
Synonyms:Transcription initiation factor TFIID subunit 4B (Transcription initiation factor TFIID 105 kDa subunit) (TAF(II)105) (TAFII-105) (TAFII105), TAF4B, TAF2C2 TAFII105
UniProt Protein Function:TAF4B: Cell type-specific subunit of TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAFII105 is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Belongs to the TAF4 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: nucleolus; nucleoplasm

Molecular Function:DNA binding; NF-kappaB binding

Biological Process: RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter

Disease: Spermatogenic Failure 13

NCBI Summary:TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
UniProt Code:Q92750
NCBI GenInfo Identifier:119370533
NCBI Gene ID:6875
NCBI Accession:Q92750.2
UniProt Secondary Accession:Q92750,Q29YA4, Q29YA5,
UniProt Related Accession:Q92750
Molecular Weight:63,364 Da
NCBI Full Name:Transcription initiation factor TFIID subunit 4B
NCBI Synonym Full Names:TATA-box binding protein associated factor 4b
NCBI Official Symbol:TAF4B  
NCBI Official Synonym Symbols:SPGF13; TAF2C2; TAFII105  
NCBI Protein Information:transcription initiation factor TFIID subunit 4B
UniProt Protein Name:Transcription initiation factor TFIID subunit 4B
UniProt Synonym Protein Names:Transcription initiation factor TFIID 105 kDa subunit; TAF(II)105; TAFII-105; TAFII105
Protein Family:Transcription initiation factor
UniProt Gene Name:TAF4B  
UniProt Entry Name:TAF4B_HUMAN
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Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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