The TAF1 Polyclonal Antibody (CAB15730) is a crucial tool for researchers studying TAF1, a key protein involved in transcription initiation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the TAF1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential component for studies in molecular biology and gene regulation.TAF1 is a core subunit of the TFIID complex, which is essential for the initiation of transcription by RNA polymerase II. Dysregulation of TAF1 has been linked to various diseases, including neurodevelopmental disorders and cancer.
As such, understanding the function of TAF1 is crucial for unraveling the molecular mechanisms underlying these conditions and developing potential therapeutic interventions.By utilizing the TAF1 Polyclonal Antibody (CAB15730), researchers can gain valuable insights into the role of TAF1 in gene expression and its implications for human health. This antibody serves as a reliable tool for investigating TAF1 function and conducting in-depth studies on transcriptional regulation, offering new opportunities for breakthrough discoveries in the field of molecular biology.
Product Name:
TAF1 Rabbit Polyclonal Antibody
SKU:
CAB15730
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 990-1250 of human TAF1 (NP_620278.1).
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons.
Purification Method:
Affinity purification
Gene ID:
6872
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TAF1 Rabbit pAb (CAB15730) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
