The TAAR5 Polyclonal Antibody (PACO55798) is a valuable tool for researchers studying TAAR5, a member of the trace amine-associated receptor (TAAR) family involved in olfactory signal transduction. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards TAAR5 in human samples, making it ideal for use in Western blot and immunohistochemistry applications.TAAR5 is known for its role in detecting and responding to volatile amines, chemicals that play a key role in odor perception.
Research on TAAR5 is important for understanding the molecular mechanisms underlying smell sensation and could lead to insights into olfactory disorders and therapeutic interventions. The TAAR5 Polyclonal Antibody enables precise detection and localization of TAAR5 in various tissues, offering researchers a valuable tool for advancing studies in neuroscience and sensory biology.
Western Blot. Positive WB detected in: Rat brain tissue, Mouse brain tissue. All lanes: TAAR5 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 kDa. Observed band size: 39 kDa.
Immunofluorescence staining of Hela cells with PACO55798 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO55798 diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Olfactory receptor specific for trimethylamine, a trace amine. Also activated at lower level by dimethylethylamine. Trimethylamine is a bacterial metabolite found in some animal odors, and to humans it is a repulsive odor associated with bad breath and spoiled food. This receptor is probably mediated by the G(s)-class of G-proteins which activate adenylate cyclase.
TAAR5: Orphan receptor. Ligands are likely small molecules, either sharing some similarities with trace amine as, e.g. derivatives of indolamines (such as 5-methoxytryptamine) or of phenylethylamines (such as phenylethanolamine) or being any kind of metabolite of amino acids or biogenic amine neurotransmitters. Belongs to the G-protein coupled receptor 1 family.Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1Chromosomal Location of Human Ortholog: 6q23Cellular Component: integral to membrane; integral to plasma membraneMolecular Function: G-protein coupled receptor activityBiological Process: signal transduction
