The T (Testosterone) ELISA Kit from Assay Genie is designed for the quantitative detection of Testosterone levels in various biological samples.
Testosterone is a vital androgen hormone that plays a key role in numerous physiological processes, including muscle mass and strength, fat distribution, bone density, and male reproductive tissues. Accurate detection and measurement of Testosterone is critical to research in endocrinology, reproductive health, athletic performance, and age-related studies. With Assay Genie's T (Testosterone) ELISA Kit, you can expect exceptional sensitivity and specificity, ensuring reliable and reproducible results. This kit is manufactured under strict quality control standards, offering robust performance and easy-to-use instructions. Therefore, it is an excellent choice for both research and clinical applications where dependable hormone quantification is needed. Choose Assay Genie's T (Testosterone) ELISA Kit for precise and reliable measurement of this critical hormone in your research.
Product Name:
T (Testosterone) ELISA Kit
SKU:
AEES00014
Target:
T (Testosterone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.17 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with T. During the reaction, T in the sample or standard competes with a fixed amount of T on the solid phase supporter for sites on the Biotinylated Detection Ab specific to T. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of T in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92
86
87
Average (%)
87-102
87-115
84-94
1:4
Range (%)
91
98
88
Average (%)
83-90
88-108
85-101
1:8
Range (%)
85
96
93
Average (%)
80-95
87-95
80-102
1:16
Range (%)
88
89
92
Average (%)
89-100
82-89
83-96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
80-90
84
EDTA plasma (n=5)
82-94
87
Cell culture media (n=5)
85-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.33
5.22
10.88
1.54
5.73
10.02
Standard deviation
0.11
0.35
0.46
0.14
0.35
0.68
C V (%)
8.13
6.72
4.27
9.23
6.18
6.75
Sample type &Sample volume:
serum, plasma, urine, saliva; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum, plasma, urine, saliva.
Specificity:
This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed.
