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SYT1 Polyclonal Antibody (CAB24840)

SKU:
CAB24840
$155
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Description

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Product Name:SYT1 Polyclonal Antibody
SKU:CAB24840
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SYT1(NP_005630.1).
Sequence:MVSE SHHE ALAA PPVT TVAT VLPS NATE PASP GEGK EDAF SKLK EKFM NELH KIPL PPWA LIAI AIVA VLLV LTCC FCIC KKCL FKKK NKKK GKEK GGKN
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:SYT1; P65; SVP65; SYT; synaptotagmin-1
Positive Sample:Mouse brain
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Cytoplasmic vesicle, Single-pass membrane protein, chromaffin granule membrane, secretory vesicle, synaptic vesicle membrane.
Calculated MW:47kDa
Observed MW:60kDa

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).

Purification Method:Affinity purification
Gene ID:6857
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Mouse brain using SYT1 Rabbit pAb(CAB24840) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.

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