The Synaptophysin Polyclonal Antibody (PAC021458) is a valuable tool for researchers studying synaptophysin, a protein found in synaptic vesicles that plays a crucial role in neurotransmission. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in immunohistochemistry (IHC) and immunofluorescence (IF) applications. By targeting synaptophysin, researchers can accurately detect and analyze this protein in various tissues and cell types, making it an essential component in neuroscience and neurobiology research.
Synaptophysin is a key marker for synaptic vesicles and is widely used in studies related to neurodevelopment, neurodegenerative diseases, and synaptic plasticity. Its involvement in neurotransmission and synaptic function make it a valuable target for investigating conditions such as Alzheimer's disease, Parkinson's disease, and epilepsy. By understanding the role of synaptophysin, researchers can gain insights into the mechanisms underlying brain disorders and potentially develop therapeutic interventions targeting synaptic dysfunction.
Antibody Name:
Synpr Antibody (PACO21458)
Antibody SKU:
PACO21458
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:
Mouse, Rat
Immunogen:
Peptide sequence around aa.257~261(G-P-T-S-F)derived from Rat synaptophysin 2.
Form:
Liquid
Storage Buffer:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extract from rat brain and mouse brain using synaptophysin 2 Antibody.
Background:
Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).
Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4. Valtorta, F. et al. (2004) Bioessays 26, 445-53. Arthur, C.P. and Stowell, M.H. (2007) Structure 15, 707-14. Bonanomi, D. et al. (2007) Biochem J 404, 525-34.
Synonyms:
Synpr
UniProt Protein Function:
SYNPR: Intrinsic membrane protein of small synaptic vesicles. Probable vesicular channel protein. Belongs to the synaptophysin/synaptobrevin family.Protein type: Membrane protein, multi-pass; Membrane protein, integralCellular Component: neuron projection; synaptic vesicle membrane; cell junction; integral to synaptic vesicle membraneMolecular Function: transporter activityBiological Process: transport
UniProt Protein Details:
NCBI Summary:
synaptic vesicle membrane protein; member of the synaptophysin family [RGD, Feb 2006]
