The Symplekin Polyclonal Antibody (CAB8722) is a valuable tool for researchers studying Symplekin, an essential protein involved in various cellular processes. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is suitable for use in Western blot applications. By binding to the Symplekin protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and cell signaling.Symplekin plays a crucial role in the regulation of gene expression and the assembly of the pre-initiation complex during transcription.
Its involvement in these processes makes it a key player in cellular function and has implications for research in areas such as cancer, developmental biology, and gene regulation. By understanding the function and regulation of Symplekin, researchers can gain valuable insights into the mechanisms underlying cellular processes and potentially develop targeted therapies for diseases related to its dysregulation.
Product Name:
SYMPK Rabbit Polyclonal Antibody
SKU:
CAB8722
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 310-530 of human SYMPK (NP_004810.2).
This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.
Purification Method:
Affinity purification
Gene ID:
8189
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SYMPK antibody (CAB8722) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
