The Symmetric Dimethyl Histone H4 (R3) Polyclonal Antibody (CAB3159) is a valuable tool for researchers studying histone modifications and epigenetic regulation. This antibody, generated in rabbits, specifically targets the symmetric dimethylation of histone H4 at arginine 3 (R3) and is highly reactive with human samples. Validated for use in Western blot applications, this antibody enables the detection and analysis of histone H4 modifications in various cell types.Histone modifications, such as symmetric dimethylation, play a crucial role in gene regulation and chromatin structure, impacting processes like transcription and DNA repair.
The detection of histone modifications like symmetric dimethyl histone H4 (R3) can provide insights into epigenetic mechanisms underlying diseases such as cancer, neurological disorders, and developmental abnormalities.By utilizing the Symmetric Dimethyl Histone H4 (R3) Polyclonal Antibody (CAB3159), researchers can further explore the role of histone modifications in chromatin dynamics and gene expression, leading to a better understanding of epigenetic regulation and potential therapeutic targets for various diseases.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (CAB3159) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
