The Symmetric Dimethyl Histone H3 (R2) Polyclonal Antibody (CAB2373) is a valuable tool for researchers studying epigenetics and chromatin regulation. This antibody, produced in rabbits, is highly specific to the histone H3 modification at arginine 2 and is validated for use in various applications, including Western blot and immunofluorescence.Histone modifications, such as symmetric dimethylation of histone H3 at arginine 2, play a crucial role in gene expression regulation and chromatin structure. Dysregulation of these modifications has been linked to various diseases, including cancer and developmental disorders.
The CAB2373 antibody allows for the detection and analysis of this specific histone modification, providing researchers with key insights into the mechanisms underlying these diseases.By utilizing the CAB2373 antibody, researchers can investigate the role of symmetric dimethyl histone H3 (R2) in various cellular processes, including transcriptional regulation and DNA repair. This antibody is an essential tool for scientists working in the fields of epigenetics, cancer biology, and developmental biology, offering precise detection and analysis of this important histone modification.
HeLa acid extract,NIH/3T3 acid extract,C6 acid extract,H3 protein
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
16kDa
Observed MW:
17kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Symmetric DiMethyl-Histone H3-R2 Rabbit pAb (CAB2373) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
