The Symmetric Dimethyl-Histone H3 (R17) Polyclonal Antibody (CAB3152) is a valuable tool for researchers studying histone modifications and chromatin structure. This antibody, raised in rabbits, specifically targets the symmetric dimethylated form of histone H3 at arginine 17, a modification linked to gene regulation and chromatin organization. Validated for use in various applications such as Western blot and immunofluorescence, this antibody offers researchers the ability to detect and analyze the presence of symmetric dimethyl-histone H3 (R17) in human samples. With its high reactivity and specificity, this antibody is ideal for investigating epigenetic mechanisms in cellular processes, developmental biology, and disease states such as cancer.
Understanding the role of histone modifications like symmetric dimethyl-H3 (R17) is essential for unraveling the complexities of gene expression regulation and chromatin dynamics. By utilizing this antibody, researchers can further explore the intricate relationship between chromatin modifications and various biological processes, leading to potential advancements in epigenetic-based therapeutics and precision medicine.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Symmetric DiMethyl-Histone H3-R17 Rabbit pAb (CAB3152) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
