The SUPT5H Polyclonal Antibody (CAB9193) is a valuable tool for researchers studying SUPT5H, a protein involved in transcriptional regulation and elongation. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the SUPT5H protein, researchers can detect and analyze its expression in a variety of cell types, making it ideal for studies in molecular biology and gene expression.SUPT5H is a key factor in the regulation of RNA polymerase II activity, playing a crucial role in the transcriptional elongation process.
Its involvement in gene expression modulation makes it a significant target for research in fields such as cancer biology, developmental biology, and epigenetics. Understanding the functions of SUPT5H can provide insights into various cellular processes and contribute to the development of therapeutic interventions targeting gene expression pathways.Overall, the SUPT5H Polyclonal Antibody (CAB9193) offers researchers a reliable tool for investigating the roles and mechanisms of SUPT5H in cellular processes, offering potential applications in both basic research and drug discovery efforts.
Product Name:
SUPT5H/SPT5 Rabbit Polyclonal Antibody
SKU:
CAB9193
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 125-425 of human SUPT5H/SPT5 (NP_003160.2).
WB,1:200 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
SPT5; SPT5H; Tat-CT1; SUPT5H/SPT5
Positive Sample:
U-87MG,HeLa,A-549,Mouse liver
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
121kDa
Observed MW:
160kDa
Enables enzyme binding activity and protein heterodimerization activity. Involved in positive regulation of macroautophagy; regulation of RNA metabolic process; and transcription elongation from RNA polymerase II promoter. Located in nucleoplasm. Part of DSIF complex.
Purification Method:
Affinity purification
Gene ID:
6829
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using SUPT5H/SPT5 Rabbit pAb (CAB9193) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
