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Superoxide Anionic Assay Kit - Colorimetric (WST-1 Method) (MAES0014)

SKU:
MAES0014
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Urine, Cells, Cell Culture Supernatant, Leucocyte
Assay Time:
35 min
Shipping:
Gel Pack
Research Area:
Oxidative Stress
Reactive Oxygen Species
$491
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Superoxide Anionic Assay Kit - Colorimetric (WST-1 Method)
Product Code:MAES0014
Product Size:96 Assays
Sample Type:Serum, plasma, urine, cells, cell culture supernatant, leucocyte
Assay Time:35 min
Instrument:Microplate reader
Recovery Rate:100
Inter CV:5.8
Inter CV:2

Superoxide anion radical is a kind of reactive oxygen, which is formed by the reduction of molecular oxygen. Excessive accumulation of reactive oxygen species will lead to oxidative stress.

Superoxide anion free radicals are produced through the reaction system of xanthine and xanthine oxidase. WST-1 (a water-soluble tetrazolium salt) can react with the generated superoxide anion to produce water-soluble formazan. When the tested sample contains the superoxide anion free radical inhibitor, it can inhibit the formation of formazan. When the tested sample contains the substance that produces superoxide anion free radical, it can promote the formation of formazan dye. By colorimetric analysis of WST-1 products, the units of activity of inhibition or production of superoxide anion radical in samples can be calculated.

Shelf Life:6 Months
Storage:Reagent 3:-20°C;, Others:2-8°C
Shipping:Gel Pack
Research Area:Oxidative stress

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