Anti-SUMO1 Antibody (CAB2130)
- SKU:
- CAB2130
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
Anti-SUMO1 Antibody (CAB2130)
The Sumo1 Polyclonal Antibody (CAB2130) is a valuable tool for researchers studying the SUMO1 protein, a small ubiquitin-like protein involved in post-translational modification. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.SUMO1 plays a crucial role in regulating various cellular processes, including protein localization, protein stability, and protein-protein interactions. By binding specifically to the SUMO1 protein, this antibody allows for the detection and analysis of SUMO1 in different cell types, making it well-suited for studies in molecular biology, cell biology, and signal transduction pathways.
The importance of SUMO1 in cellular function and disease makes it a key target for research in areas such as cancer biology, neurodegenerative disorders, and viral infections. Investigating the role of SUMO1 can provide valuable insights into disease mechanisms and potentially lead to the development of novel therapeutic strategies. The Sumo1 Polyclonal Antibody is a reliable tool for researchers looking to explore the functions of SUMO1 in various biological contexts.
| Antibody Name: | Anti-SUMO1 Antibody |
| Antibody SKU: | CAB2130 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human SUMO1 (NP_003343.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human SUMO1 (NP_003343.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSDQ EAKP STED LGDK KEGE YIKL KVIG QDSS EIHF KVKM TTHL KKLK ESYC QRQG VPMN SLRF LFEG QRIA DNHT PKEL GMEE EDVI EVYQ EQTG GHST V |
| Gene ID: | 7341 |
| Uniprot: | P63165 |
| Cellular Location: | Cell membrane, Cytoplasm, Nucleus, Nucleus membrane, Nucleus speckle, PML body |
| Calculated MW: | 8kDa/11kDa |
| Observed MW: | 80KDa |
| Synonyms: | DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1, SUMO1, SUMO-1 |
| Background: | This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. |
| UniProt Protein Function: | SUMO1: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Interacts with SAE2, UBE2I, RANBP2, PIAS1 and PIAS2. Interacts with PARK2. Covalently attached to a number of proteins such as IKFZ1, PML, RANGAP1, HIPK2, SP100, p53, p73-alpha, MDM2, JUN, DNMT3B and TDG. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, EXOSC9, RAD51 and RAD52. Interacts with USP25 (via ts SIM domain); the interaction weakly sumoylates USP25. Belongs to the ubiquitin family. SUMO subfamily. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 2q33 Cellular Component: nucleoplasm; PML body; nuclear membrane; dendrite; cytoplasm; nucleolus; fibrillar center; synapse; nuclear speck; nuclear pore; nucleus Molecular Function:protein binding; ubiquitin protein ligase binding; transcription factor binding; SUMO ligase activity Biological Process: negative regulation of transcription factor activity; cytokine and chemokine mediated signaling pathway; PML body organization and biogenesis; palate development; post-translational protein modification; DNA repair; negative regulation of DNA binding; regulation of protein localization; positive regulation of protein complex assembly; cellular protein metabolic process; protein sumoylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of transcription, DNA-dependent Disease: Orofacial Cleft 10 |
| NCBI Summary: | This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P63165 |
| NCBI GenInfo Identifier: | 52783799 |
| NCBI Gene ID: | 7341 |
| NCBI Accession: | P63165.1 |
| UniProt Secondary Accession: | P63165,P55856, Q6FGG0, Q6NZ62, Q93068, A8MUS8, B2R4I5 |
| UniProt Related Accession: | P63165 |
| Molecular Weight: | 101 |
| NCBI Full Name: | Small ubiquitin-related modifier 1 |
| NCBI Synonym Full Names: | small ubiquitin-like modifier 1 |
| NCBI Official Symbol: | SUMO1Â Â |
| NCBI Official Synonym Symbols: | DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3Â Â |
| NCBI Protein Information: | small ubiquitin-related modifier 1; sentrin; SMT3 homolog 3; GAP modifying protein 1; ubiquitin-like protein UBL1; ubiquitin-like protein SMT3C; SMT3 suppressor of mif two 3 homolog 1; ubiquitin-homology domain protein PIC1 |
| UniProt Protein Name: | Small ubiquitin-related modifier 1 |
| UniProt Synonym Protein Names: | GAP-modifying protein 1; GMP1; SMT3 homolog 3; Sentrin; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Smt3C; Ubiquitin-like protein UBL1 |
| Protein Family: | Small ubiquitin-related modifier |
| UniProt Gene Name: | SUMO1Â Â |
| UniProt Entry Name: | SUMO1_HUMAN |
 | Western blot analysis of extracts of 293T cells, using SUMO1 antibody (CAB2130) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using SUMO1 antibody (CAB2130) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using SUMO1 antibody (CAB2130) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using SUMO1 antibody (CAB2130) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of C6 cells using SUMO1 antibody (CAB2130) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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