The SUCLA2 Polyclonal Antibody (CAB10040) is a valuable tool for researchers studying SUCLA2, a key enzyme involved in mitochondrial metabolism. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the SUCLA2 protein, this antibody enables the detection and analysis of SUCLA2 in a variety of cell types, making it ideal for investigations in biochemistry and molecular biology.SUCLA2 is a crucial enzyme that plays a central role in the citric acid cycle, where it is involved in the conversion of succinyl-CoA to succinate.
Dysregulation of SUCLA2 has been linked to various metabolic disorders and mitochondrial diseases, making it an important target for research in fields such as metabolism, genetics, and bioenergetics. Understanding the function and regulation of SUCLA2 is essential for unraveling its role in human health and disease, potentially leading to the development of novel therapeutic strategies.
Product Name:
SUCLA2 Rabbit Polyclonal Antibody
SKU:
CAB10040
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human SUCLA2 (NP_003841.1).
Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
8803
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SUCLA2 antibody (CAB10040) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
