The Sub1 Polyclonal Antibody (CAB7070) is a valuable tool for researchers studying Sub1, a protein involved in various cellular processes such as transcription and DNA repair. This antibody, developed using a rabbit host, exhibits high specificity for human samples and is validated for use in Western blotting applications. By binding to the Sub1 protein, researchers can accurately detect and analyze its expression in different cell types, making it an essential component for studies in molecular biology and cancer research.
Sub1, also known as PC4, plays a crucial role in regulating gene expression and maintaining genomic stability. Its involvement in transcriptional activation and DNA damage repair processes makes it a promising target for understanding diseases such as cancer and genetic disorders. By unraveling the mechanisms through which Sub1 operates, researchers can potentially uncover new therapeutic avenues for treating these conditions.
Product Name:
SUB1 Rabbit Polyclonal Antibody
SKU:
CAB7070
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human SUB1 (NP_006704.3).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
P15; PC4; p14; SUB1
Positive Sample:
HL-60,BT-474,SW620,Jurkat,Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
14kDa
Observed MW:
17kDa
Enables identical protein binding activity; single-stranded DNA binding activity; and transcription coactivator activity. Involved in negative regulation of DNA metabolic process and regulation of transcription by RNA polymerase II. Located in nucleolus and nucleoplasm. Part of transcription regulator complex.
Purification Method:
Affinity purification
Gene ID:
10923
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using SUB1 Rabbit pAb (CAB7070) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
