The STT3A Antibody (PAC030190) is a highly specific antibody designed for research involving the STT3A protein. STT3A is a crucial subunit of the oligosaccharyltransferase complex, which is responsible for protein N-glycosylation in the endoplasmic reticulum. This antibody, raised in rabbits, is optimized for use in various applications including Western blotting and immunofluorescence.The STT3A Antibody allows for the detection and analysis of STT3A protein levels in human samples, making it an invaluable tool for studying protein glycosylation processes.
The accurate identification of STT3A in different cell types provides researchers with a deeper understanding of how glycosylation impacts cellular function and disease pathology.This antibody is essential for investigations into protein glycosylation disorders, such as congenital disorders of glycosylation (CDG), as well as for exploring the role of N-glycosylation in cancer progression and immune response modulation. By targeting STT3A, researchers can uncover new therapeutic targets and develop innovative treatments for glycosylation-related diseases.
Antibody Name:
STT3A Antibody (PACO30190)
Antibody SKU:
PACO30190
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A protein (39-114AA)
Immunohistochemistry of paraffin-embedded human pancreatic cancer using PACO30190 at dilution of 1:100.
Background:
Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient cotranslational glycosylation and mediate glycosylation of sites that have been skipped by STT3A.
ITM1: Catalytic component of oligosaccharyltransferase (OST) oligosaccharyltransferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X- Ser/Thr consensus motif in nascent polypeptide chains. N- glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient cotranslational glycosylation and mediate glycosylation of sites that have been skipped by STT3A. Belongs to the STT3 family.Protein type: Membrane protein, multi-pass; EC 2.4.99.18; Membrane protein, integral; Endoplasmic reticulum; TransferaseChromosomal Location of Human Ortholog: 11q23.3Cellular Component: endoplasmic reticulum membrane; membrane; oligosaccharyl transferase complexMolecular Function: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; protein bindingBiological Process: co-translational protein modification; post-translational protein modification; protein amino acid N-linked glycosylation via asparagineDisease: Congenital Disorder Of Glycosylation, Type Iw
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a catalytic subunit of the N-oligosaccharyltransferase (OST) complex, which functions in the endoplasmic reticulum to transfer glycan chains to asparagine residues of target proteins. A separate complex containing a similar catalytic subunit with an overlapping function also exists. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
