[KO Validated] STRADA Rabbit Polyclonal Antibody (CAB18012)
The STRADA Polyclonal Antibody (CAB18012) is a valuable tool for researchers studying the STRADA protein, a key regulator of the MAPK signaling pathway. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the STRADA protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component of studies in cell signaling and cancer research.The STRADA protein is crucial for regulating cell growth and proliferation through its role in the MAPK signaling pathway.
Dysregulation of this pathway has been linked to various types of cancer, making the STRADA protein a promising target for therapeutic interventions. By gaining a better understanding of STRADA's function and regulation, researchers can uncover new insights into cancer biology and potentially develop novel treatment strategies. The STRADA Polyclonal Antibody is a valuable tool for unraveling the complexities of the MAPK signaling pathway and its implications for cancer biology.
Product Name:
[KO Validated] STRADA Rabbit Polyclonal Antibody
SKU:
CAB18012
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human STRADA (NP_699166.2).
LYK5; PMSE; Stlk; STRAD; NY-BR-96; STRAD alpha; DA
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
48kDa
Observed MW:
48kDa
The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known.
Purification Method:
Affinity purification
Gene ID:
92335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and STRADA knockout (KO) HeLa cells, using [KO Validated] STRADA Rabbit pAb (CAB18012) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
