The STRA8 Polyclonal Antibody (PAC061634) is a valuable tool for researchers studying the role of STRA8, a protein involved in meiosis and spermatogenesis. This antibody, produced in rabbits, shows high reactivity with human STRA8 samples and has been validated for use in Western blot applications. By binding specifically to the STRA8 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for studies in reproductive biology and fertility research.
STRA8 is essential for the process of meiotic initiation, playing a key role in germ cell development and fertility. Dysfunction of STRA8 has been associated with infertility and reproductive disorders, making it a target of interest for researchers studying male and female reproductive health. Understanding the function of STRA8 is crucial for uncovering the mechanisms underlying these conditions and developing potential treatments or interventions.
Western Blot. Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate. All lanes: STRA8 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37 kDa. Observed band size: 60 kDa.
IHC image of PACO61634 diluted at 1:500 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Meiosis-inducer required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase. During spermatogenesis, next to its role in meiotic initiation, promotes (but is not required for) spermatogonial differentiation. Can associate with DNA (possibly in an indirect manner), and in vitro can activate DNA transcription (By similarity).
Synonyms:
Stimulated by retinoic acid, gene 8 protein homolog, STRA8
UniProt Protein Function:
STRA8: Meiosis-inducer required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase.Chromosomal Location of Human Ortholog: 7q33Cellular Component: cytoplasm; nucleusMolecular Function: protein dimerization activityBiological Process: fertilization; meiotic cell cycle DNA replication checkpoint; ovarian follicle development; meiotic chromosome condensation; meiotic recombination; synapsis; meiotic DNA double-strand break formation; spermatogenesis; regulation of organ growth; female meiosis sister chromatid cohesion; DNA replication; oocyte development
UniProt Protein Details:
NCBI Summary:
This gene encodes a retinoic acid-responsive protein. A homologous protein in mouse has been shown to be involved in the regulation of meiotic initiation in both spermatogenesis and oogenesis, though feature differences between the mouse and human proteins suggest that these homologs are not entirely functionally equivalent. It is thought that this gene may play a role in spermatogenesis in humans. [provided by RefSeq, Nov 2010]
