The STON1 Antibody (PAC021999) is a polyclonal antibody designed for research involving STON1, a protein involved in vesicle trafficking and autophagy. This antibody is raised in rabbits and is highly reactive with human samples. It is validated for use in Western blot applications and binds specifically to the STON1 protein, allowing for detection and analysis in various cell types.STON1 is a key player in the process of autophagy, a cellular mechanism involved in recycling damaged organelles and proteins to maintain cellular homeostasis. Dysregulation of autophagy has been implicated in various diseases, including cancer and neurodegenerative disorders.
Therefore, studying the function of STON1 and its role in autophagy can provide valuable insights into disease mechanisms and potential therapeutic targets.The STON1 Antibody is an essential tool for researchers exploring the role of STON1 in vesicle trafficking and autophagy. Its high specificity and reactivity with human samples make it a reliable reagent for immunological and cancer research studies. By understanding the function of STON1, researchers can unravel the complexities of autophagy and its implications in disease pathogenesis, paving the way for the development of novel therapeutic interventions.
Antibody Name:
STON1 Antibody (PACO21999)
Antibody SKU:
PACO21999
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from C-terminal of human STON1.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from K562 cells, using STON1 antibody.
Background:
May be involved in the endocytic machinery By similarity.
STON1: May be involved in the endocytic machinery. Belongs to the Stoned B family.Chromosomal Location of Human Ortholog: 2p16.3Cellular Component: clathrin adaptor complexBiological Process: intracellular protein transport; regulation of endocytosis; endocytosis
UniProt Protein Details:
NCBI Summary:
Endocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. This gene encodes one of two human homologs of the Drosophila melanogaster stoned B protein. This protein is related to components of the endocytic machinery and exhibits a modular structure consisting of an N-terminal proline-rich domain, a central region of homology specific to the human stoned B-like proteins, and a C-terminal region homologous to the mu subunits of adaptor protein (AP) complexes. Read-through transcription of this gene into the neighboring downstream gene, which encodes TFIIA-alpha/beta-like factor, generates a transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2010]
