The STIM1 Polyclonal Antibody (CAB7411) is a valuable tool for researchers studying the STIM1 protein, a key regulator of calcium signaling pathways. This antibody, generated in rabbits, demonstrates high specificity and sensitivity for STIM1 in human samples, making it a reliable choice for Western blot applications. By specifically binding to STIM1, this antibody enables accurate detection and analysis of the protein in various cell types, facilitating research in immunology and cell signaling pathways.STIM1 is a crucial component of the store-operated calcium entry (SOCE) pathway, playing a vital role in cellular functions such as gene expression, cell proliferation, and immune response.
Dysregulation of STIM1 signaling has been implicated in numerous diseases, including cancer, autoimmune disorders, and neurological conditions. Understanding the mechanisms of STIM1 activation and its downstream effects is essential for developing targeted therapies for these diseases.By using the STIM1 Polyclonal Antibody (CAB7411), researchers can delve deeper into the intricate functions of the STIM1 protein and its implications in various disease states. This antibody offers a reliable tool for exploring the role of STIM1 in calcium signaling pathways and its potential as a therapeutic target in the treatment of disease.
Product Name:
STIM1 Rabbit Polyclonal Antibody
SKU:
CAB7411
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 530-685 of human STIM1 (NP_003147.2).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
GOK; TAM; TAM1; IMD10; STRMK; D11S4896E; STIM1
Positive Sample:
K-562,HeLa,Jurkat,MCF7
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Single-pass type I membrane protein, cytoskeleton.
Calculated MW:
77kDa
Observed MW:
85kDa
This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6786
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using STIM1 Rabbit pAb (CAB7411) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
