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STAT4 (Phospho-Tyr693) Colorimetric Cell-Based ELISA Kit

SKU:
CBCAB00223
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human
Mouse
Rat
Detection Method:
Colorimetric
€599
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Description

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STAT4 (Phospho-Tyr693)Colorimetric Cell-Based ELISA Kit

The STAT4 Phospho-Tyr693 Colorimetric Cell-Based ELISA Kit is a cutting-edge research tool designed for the precise and reliable detection of phosphorylated STAT4 at tyrosine 693 in cell lysates. This innovative kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications.Phosphorylated STAT4 at tyrosine 693 is a key signaling molecule involved in the regulation of immune responses and inflammatory processes. Dysregulation of STAT4 phosphorylation has been implicated in various autoimmune diseases, including rheumatoid arthritis and multiple sclerosis, making it a valuable target for research and drug development.

With the STAT4 Phospho-Tyr693 Colorimetric Cell-Based ELISA Kit, researchers can easily quantify phosphorylated STAT4 levels in cell lysates, providing valuable insights into the mechanisms underlying immune-mediated diseases and potential therapeutic targets. Trust in the accuracy and precision of this advanced ELISA kit for your research needs.

Product Name:STAT4 (Phospho-Tyr693) Colorimetric Cell-Based ELISA
Product Code:CBCAB00223
ELISA Type:Cell-Based
Target:STAT4 (Phospho-Tyr693)
Reactivity:Human, Mouse, Rat
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The STAT4 (Phospho-Tyr693) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect STAT4 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated STAT4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on STAT4 phosphorylation.

Qualitative determination of STAT4 (Phospho-Tyr693) concentration is achieved by an indirect ELISA format. In essence, STAT4 (Phospho-Tyr693) is captured by STAT4 (Phospho-Tyr693)-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 6775, UniProt ID: Q14765, OMIM: 180300/600558/612253, Unigene: Hs.80642
Gene Symbol:STAT4
Sub Type:Phospho
UniProt Protein Function:STAT4: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q32.2-q32.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function:protein binding; signal transducer activity; sequence-specific DNA binding; transcription factor activity

Biological Process: cell proliferation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; JAK-STAT cascade; protein amino acid phosphorylation

Disease: Systemic Lupus Erythematosus, Susceptibility To, 11

NCBI Summary:The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]
UniProt Code:Q14765
NCBI GenInfo Identifier:6226158
NCBI Gene ID:6775
NCBI Accession:Q14765.1
UniProt Secondary Accession:Q14765,Q96NZ6,
UniProt Related Accession:Q14765
Molecular Weight:85,941 Da
NCBI Full Name:Signal transducer and activator of transcription 4
NCBI Synonym Full Names:signal transducer and activator of transcription 4
NCBI Official Symbol:STAT4  
NCBI Official Synonym Symbols:SLEB11  
NCBI Protein Information:signal transducer and activator of transcription 4
UniProt Protein Name:Signal transducer and activator of transcription 4
Protein Family:Protein slender lobes
UniProt Gene Name:STAT4  
UniProt Entry Name:STAT4_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-STAT4 (Phospho-Tyr693) Antibody, Anti-STAT4 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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