STAT1 Rabbit Polyclonal Antibody (CAB12075)
- SKU:
- CAB12075
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Product Name: | STAT1 Rabbit Polyclonal Antibody |
| SKU: | CAB12075 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 650-750 of human STAT1 (NP_009330.1). |
| Sequence: | NYKV MAAE NIPE NPLK YLYP NIDK DHAF GKYY SRPK EAPE PMEL DGPK GTGY IKTE LISV SEVH PSRL QTTD NLLP MSPE EFDE VSRI VGSV EFDS MMNT V |
| Tested Applications: | WB IHC-P IF/ICC IP ELISA |
| Recommended Dilution: | WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Synonyms: | CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91; STAT1 |
| Positive Sample: | HeLa,Mouse spleen,Rat spleen |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Nucleus. |
| Calculated MW: | 87kDa |
| Observed MW: | 91kDa |
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.
| Purification Method: | Affinity purification |
| Gene ID: | 6772 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from HeLa cells, using STAT1 Rabbit pAb (CAB12075) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s. |
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