The STAM Polyclonal Antibody (CAB4198) is a valuable tool for researchers studying the STAM protein, a key component of the endosomal sorting complex required for transport (ESCRT) pathway. This antibody, generated in rabbits, is highly specific to STAM and has been validated for use in various applications, including Western blot and immunofluorescence.STAM is essential for the sorting of ubiquitinated cargo proteins into multivesicular bodies for degradation, playing a crucial role in endosomal trafficking and membrane dynamics. Dysregulation of the ESCRT pathway and STAM has been implicated in various diseases, including cancer, viral infections, and neurodegenerative disorders.
By targeting STAM with this polyclonal antibody, researchers can gain insight into the molecular mechanisms underlying these pathologies and potentially identify new therapeutic targets.With its high reactivity and specificity, the STAM Polyclonal Antibody is an excellent tool for investigating the function of STAM in cellular processes and diseases, making it a valuable asset for research in cell biology, molecular biology, and biomedicine.
Product Name:
STAM Rabbit Polyclonal Antibody
SKU:
CAB4198
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 120-210 of human STAM (NP_003464.1).
Cytoplasm, Cytoplasmic side, Early endosome membrane, Peripheral membrane protein.
Calculated MW:
59kDa
Observed MW:
70kDa
This gene encodes a member of the signal-transducing adaptor molecule family. These proteins mediate downstream signaling of cytokine receptors and also play a role in ER to Golgi trafficking by interacting with the coat protein II complex. The encoded protein also associates with hepatocyte growth factor-regulated substrate to form the endosomal sorting complex required for transport-0 (ESCRT-0), which sorts ubiquitinated membrane proteins to the ESCRT-1 complex for lysosomal degradation. Alternatively spliced transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
8027
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using STAM antibody (CAB4198) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
