The STAG3 Antibody (PAC021980) is a polyclonal antibody designed for research involving STAG3, a key player in meiotic chromosome organization and segregation. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot and immunofluorescence applications. It binds specifically to the STAG3 protein, enabling precise detection and analysis in various cell types, making it ideal for studies in meiosis, chromosomal dynamics, and reproductive biology.STAG3 is a crucial component of the cohesin complex, essential for proper chromosome structure and function during meiosis.
Its involvement in meiotic cell division makes it a critical target for research in infertility, reproductive disorders, and genetic diseases. Understanding the role of STAG3 in meiotic processes is vital for unraveling the mechanisms underlying chromosomal abnormalities and genetic disorders, paving the way for potential therapeutic interventions in reproductive health and genetic diseases.
Antibody Name:
STAG3 Antibody (PACO21980)
Antibody SKU:
PACO21980
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from C-terminal of human STAG3.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from Jurkat cells, using STAG3 antibody.
Background:
Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I By similarity. The German cDNA consortium; Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
STAG3: Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis- specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I. Belongs to the SCC3 family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 7q22.1Cellular Component: chromosome, pericentric region; extracellular space; nucleus; synaptonemal complexBiological Process: synaptonemal complex assemblyDisease: Premature Ovarian Failure 8
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is expressed in the nucleus and is a subunit of the cohesin complex which regulates the cohesion of sister chromatids during cell division. A mutation in this gene is associated with premature ovarian failure. Alternate splicing results in multiple transcript variants encoding distinct isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Apr 2014]
