The ST6GAL1 Polyclonal Antibody (CAB5754) is a valuable tool for researchers studying ST6GAL1, a key enzyme involved in glycosylation processes. This antibody, generated in rabbits, exhibits high specificity towards human samples and is validated for use in Western blot applications. By targeting the ST6GAL1 protein, this antibody enables detection and analysis in various cell types, making it suitable for investigations in glycobiology and cancer research.ST6GAL1, also known as ST6 beta-galactosamide alpha-2,6-sialyltransferase 1, is essential for the biosynthesis of sialylated glycoconjugates, which play critical roles in cell adhesion, signaling, and immune response modulation.
Dysregulation of ST6GAL1 expression has been implicated in various diseases, including cancer, inflammation, and neurological disorders. Therefore, studying the function of ST6GAL1 is crucial for understanding its impact on disease progression and developing targeted therapeutic strategies.
Product Name:
ST6GAL1 Rabbit Polyclonal Antibody
SKU:
CAB5754
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-200 of human ST6GAL1 (NP_775323.1).
Golgi apparatus, Golgi stack membrane, Secreted, Single-pass type II membrane protein.
Calculated MW:
47kDa
Observed MW:
50-70kDa
This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6480
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Daudi cells, using ST6GAL1 Rabbit pAb (CAB5754) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
