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ST5 Colorimetric Cell-Based ELISA (CBCAB01052)

SKU:
CBCAB01052
Product Type:
ELISA Kit
ELISA Type:
Cell Based
Reactivity:
Human
Mouse
Detection Method:
Colorimetric
€769

Description

system_update_altDatasheetMSDS

ST5 Colorimetric Cell-Based ELISA

The ST5 Colorimetric Cell-Based ELISA Kit is a cutting-edge tool that allows for the detection and quantification of specific proteins in cell samples through a colorimetric assay. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for a variety of applications.By utilizing a colorimetric detection method, this kit allows for easy and efficient measurement of protein levels in cell cultures, enabling researchers to study cellular processes and pathways with precision.

The ST5 Colorimetric Cell-Based ELISA Kit is ideal for studying protein expression, signaling pathways, and drug efficacy in cell-based research.Whether you are investigating cellular responses to stimuli, screening for potential drug candidates, or studying disease mechanisms, the ST5 Colorimetric Cell-Based ELISA Kit offers a versatile and user-friendly solution for your research needs. Invest in this innovative kit today and unlock new possibilities in cell-based research.

Product Name:ST5 Colorimetric Cell-Based ELISA
Product Code:CBCAB01052
ELISA Type:Cell-Based
Target:ST5
Reactivity:Human, Mouse
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nmStorage/Stability:4°C/6 Months
Format:96-Well Microplate

The ST5 Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect ST5 protein expression profile in cells. The kit can be used for measuring the relative amounts of ST5 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on ST5.

Qualitative determination of ST5 concentration is achieved by an indirect ELISA format. In essence, ST5 is captured by ST5-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 6764, UniProt ID: P78524, OMIM: 140750, Unigene: Hs.117715
Gene Symbol:ST5
Sub Type:None
UniProt Protein Function:DENND2B: a guanine nucleotide exchange factor (GEF) that may activate RAB9A and RAB9B. May be involved in cytoskeletal organization and tumorogenicity. Widely expressed with the exception of peripheral blood lymphocytes and lymphoid cell lines. 3 isoforms of the human protein are produced by alternative promoter. Isoform 1 interacts with the SH3 domain of ABL1, is expressed in several epithelial, fibroblast, and tumorigenic cell lines, and seems to be involved in the activation of ERK2. Isoform 3, expressed in primary cells that may be weakly tumorigenic, but not in tumorigenic cell lines, may block ERK2 activation stimulated by ABL1, and may alter cell morphology and cell growth.
UniProt Protein Details:

Protein type:GEFs; GEFs, Rab

Chromosomal Location of Human Ortholog: 11p15

Molecular Function:Rab guanyl-nucleotide exchange factor activity

NCBI Summary:This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this gene encoding distinct isoforms are identified. [provided by RefSeq, Jul 2008]
UniProt Code:P78524
NCBI GenInfo Identifier:317373507
NCBI Gene ID:6764
NCBI Accession:P78524.3
UniProt Secondary Accession:P78524,P78523, Q16492, Q7KYY2, Q7KZ12, Q8NE12, Q9BQQ6 B2R6X7, B3KXQ6,
UniProt Related Accession:P78524
Molecular Weight:70,245 Da
NCBI Full Name:Suppression of tumorigenicity 5 protein
NCBI Synonym Full Names:suppression of tumorigenicity 5
NCBI Official Symbol:ST5  
NCBI Official Synonym Symbols:HTS1; p126; DENND2B  
NCBI Protein Information:suppression of tumorigenicity 5 protein; heLa tumor suppression 1; DENN/MADD domain containing 2B
UniProt Protein Name:Suppression of tumorigenicity 5 protein
UniProt Synonym Protein Names:DENN domain-containing protein 2B; HeLa tumor suppression 1
Protein Family:Suppression of tumorigenicity 5 protein
UniProt Gene Name:ST5  
UniProt Entry Name:ST5_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies (Anti-ST5 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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