The ST5 Polyclonal Antibody (PACO6823) is a versatile research tool designed for the study of the ST5 protein, which is involved in various cellular processes including signal transduction and gene regulation. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.The ST5 antibody binds specifically to the ST5 protein, allowing for precise detection and analysis in a wide range of cell types. Its utility in research extends to fields such as molecular biology, cell signaling, and cancer research, where ST5 has been implicated in tumorigenesis and metastasis.
By targeting the ST5 protein, researchers can gain valuable insights into its role in cellular functions and disease processes, paving the way for new therapeutic approaches in cancer and other diseases. With its high specificity and sensitivity, the ST5 Polyclonal Antibody is a valuable tool for advancing scientific understanding in the field of molecular biology.
Antibody Name:
ST5 Antibody (PACO06823)
Antibody SKU:
PACO06823
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:20000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from the Internal region of human ST5.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of K562 cells using ST5 Polyclonal Antibody.
Western Blot analysis of COLO205 cells using ST5 Polyclonal Antibody.
Synonyms:
ST5; DENND2B; HTS1; Suppression of tumorigenicity 5 protein; DENN domain-containing protein 2B; HeLa tumor suppression 1
UniProt Protein Function:
DENND2B: a guanine nucleotide exchange factor (GEF) that may activate RAB9A and RAB9B. May be involved in cytoskeletal organization and tumorogenicity. Widely expressed with the exception of peripheral blood lymphocytes and lymphoid cell lines. 3 isoforms of the human protein are produced by alternative promoter. Isoform 1 interacts with the SH3 domain of ABL1, is expressed in several epithelial, fibroblast, and tumorigenic cell lines, and seems to be involved in the activation of ERK2. Isoform 3, expressed in primary cells that may be weakly tumorigenic, but not in tumorigenic cell lines, may block ERK2 activation stimulated by ABL1, and may alter cell morphology and cell growth.
This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this gene encoding distinct isoforms are identified. [provided by RefSeq, Jul 2008]
