The SSU72 Antibody (PAC040622) is a valuable tool for researchers studying RNA polymerase II transcription. This polyclonal antibody, produced in rabbits, is highly specific to SSU72 and is suitable for use in various applications including Western blot and immunohistochemistry. By binding to the SSU72 protein, this antibody enables researchers to detect and analyze its expression in different cellular contexts.SSU72 is a critical component of the RNA polymerase II complex, playing a key role in transcriptional regulation and mRNA processing. Its involvement in RNA synthesis makes it a target of interest for studies in gene expression, RNA splicing, and transcriptional elongation.
By understanding the function of SSU72, researchers can gain insights into molecular mechanisms underlying gene regulation and potentially identify novel therapeutic targets for diseases related to transcriptional dysregulation.Overall, the SSU72 Antibody (PAC040622) offers researchers a reliable tool for investigating the role of SSU72 in cellular processes, making it a valuable asset for studies in molecular biology, gene expression, and RNA metabolism.
Antibody Name:
SSU72 Antibody (PACO40622)
Antibody SKU:
PACO40622
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human RNA polymerase II subunit A C-terminal domain phosphatase SSU72 protein (1-194AA)
Immunofluorescence staining of PC-3 cells with PACO40622 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination. Plays a role in pre-mRNA polyadenylation via its interaction with SYMPK.
Synonyms:
RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (CTD phosphatase SSU72) (EC 3.1.3.16), SSU72
UniProt Protein Function:
SSU72: May be involved in the C-terminal domain of RNA polymerase II dephosphorylation, RNA processing and termination. Belongs to the SSU72 phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Phosphatase; EC 3.1.3.16Chromosomal Location of Human Ortholog: 1p36.33Cellular Component: cytoplasm; nucleusMolecular Function: protein binding; CTD phosphatase activityBiological Process: mRNA polyadenylation
