SSH1 Antibody (PACO60248)
- SKU:
- PACO60248
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
SSH1 Antibody (PACO60248)
The SSH1 Polyclonal Antibody (PACO60248) is a valuable tool for research involving SSH1, a protein involved in cell signaling pathways and actin cytoskeleton regulation. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards SSH1 in human samples, making it an excellent choice for Western blot applications. It binds specifically to the SSH1 protein, enabling accurate detection and analysis in a variety of cell types.SSH1, also known as cofilin-1, is a key regulator of actin dynamics and plays a crucial role in cell migration, invasion, and adhesion processes.
Dysregulation of SSH1 has been implicated in cancer progression, making it a promising target for anticancer therapies. By studying the function of SSH1, researchers can gain insights into its role in cancer development and potentially identify new therapeutic targets.Overall, the SSH1 Polyclonal Antibody is an essential tool for researchers studying cell signaling pathways, actin cytoskeleton dynamics, and cancer biology. Its high specificity and reliability make it a valuable asset for immunology and cancer research projects.
| Antibody Name: | SSH1 Antibody (PACO60248) |
| Antibody SKU: | PACO60248 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Protein phosphatase Slingshot homolog 1 protein (928-1049AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60248 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO60248 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein. |
| Synonyms: | Protein phosphatase Slingshot homolog 1 (EC 3.1.3.16) (EC 3.1.3.48) (SSH-like protein 1) (SSH-1L) (hSSH-1L), SSH1, KIAA1298 SSH1L |
| UniProt Protein Function: | SSH1: a dual-specificity phosphatase of the Slingshot family involved in actin reorganization. Its calcineurin-dependent activation dephosphorylates cofilin. Plays a role in later stages of mitosis by dephosphorylating and activating cofilin.Protein type: EC 3.1.3.16; Protein phosphatase, dual-specificity; EC 3.1.3.48Chromosomal Location of Human Ortholog: 12q24.11Cellular Component: cytoplasm; nucleoplasm; plasma membraneMolecular Function: actin binding; phosphoprotein phosphatase activity; protein bindingBiological Process: actin cytoskeleton organization and biogenesis; cell morphogenesis; protein amino acid dephosphorylation; regulation of actin polymerization and/or depolymerization; regulation of axonogenesis; regulation of cellular protein metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q8WYL5 |
| NCBI GenInfo Identifier: | 82582267 |
| NCBI Gene ID: | 54434 |
| NCBI Accession: | Q8WYL5.2 |
| UniProt Secondary Accession: | Q8WYL5,Q6P6C0, Q8N9A7, Q8WYL3, Q8WYL4, Q9P2P8 |
| UniProt Related Accession: | Q8WYL5 |
| Molecular Weight: | 78,978 Da |
| NCBI Full Name: | Protein phosphatase Slingshot homolog 1 |
| NCBI Synonym Full Names: | slingshot protein phosphatase 1 |
| NCBI Official Symbol: | SSH1Â Â |
| NCBI Official Synonym Symbols: | SSH1LÂ Â |
| NCBI Protein Information: | protein phosphatase Slingshot homolog 1 |
| UniProt Protein Name: | Protein phosphatase Slingshot homolog 1 |
| UniProt Synonym Protein Names: | SSH-like protein 1; SSH-1L; hSSH-1L |
| Protein Family: | Sec sixty-one protein |
| UniProt Gene Name: | SSH1Â Â |
| UniProt Entry Name: | SSH1_HUMAN |
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