The SRSF7 Antibody (CAB16738) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and reactivity with human samples, making it an ideal choice for Western blot applications. By targeting the SRSF7 protein, this antibody allows for precise detection and analysis in a variety of cell types, opening up new possibilities for research in areas such as RNA biology and cancer biology.SRSF7, also known as splicing factor 1, plays a crucial role in the intricate process of pre-mRNA splicing, influencing the generation of diverse protein isoforms and impacting gene expression patterns.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SRSF7 Antibody
SKU:
CAB16738
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
9G8, AAG3, SFRS7, SRSF7
Positive Sample:
HeLa, Mouse brain, Mouse kidney, Rat brain, Rat kidney
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
27kDa
Observed MW:
35kDa
The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an N-terminal RNA recognition motif (RRM) for binding RNA and a C-terminal RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6432
RRID
AB_2772393
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SRSF7 Rabbit pAb (CAB16738) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human esophageal using SRSF7 Rabbit pAb (CAB16738) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of U-2 OS cells using SRSF7 Rabbit pAb (CAB16738) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
