The SRRM4 Polyclonal Antibody (PACO58805) is a valuable tool for researchers studying SRRM4, a critical regulator of alternative splicing in RNA processing. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting SRRM4 protein levels in human samples, making it a reliable option for Western blot applications.SRRM4, also known as serine/arginine repetitive matrix 4, plays a key role in the splicing of pre-mRNA molecules, influencing gene expression and protein diversity. Dysregulation of alternative splicing by SRRM4 has been linked to various diseases, including cancer and neurodevelopmental disorders.
Therefore, investigating the function of SRRM4 is essential in understanding disease mechanisms and developing targeted therapies.By using the SRRM4 Polyclonal Antibody, researchers can effectively study the expression and localization of SRRM4 in different cell types, providing insights into its role in RNA processing and disease pathology. This antibody is a valuable tool for advancing research in molecular biology, oncology, and neuroscience, offering new avenues for exploring the intricate mechanisms of alternative splicing and its implications in health and disease.
Western Blot. Positive WB detected in: Rat kidney tissue, Mouse lung tissue. All lanes: SRRM4 antibody at 4.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69 kDa. Observed band size: 69 kDa.
IHC image of PACO58805 diluted at 1:500 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Splicing factor specifically required for neural cell differentiation. Acts in conjuction with nPTB/PTBP2 by binding directly to its regulated target transcripts and promotes neural-specific exon inclusion in many genes that function in neural cell differentiation. Required to promote the inclusion of neural-specific exon 10 in nPTB/PTBP2, leading to increased expression of neural-specific nPTB/PTBP2. Also promotes the inclusion of exon 16 in DAAM1 in neuron extracts (By similarity).
Synonyms:
Serine/arginine repetitive matrix protein 4 (Medulloblastoma antigen MU-MB-2.76) (Neural-specific serine/arginine repetitive splicing factor of 100 kDa) (Neural-specific SR-related protein of 100 kDa) (nSR100), SRRM4, KIAA1853
UniProt Protein Function:
Splicing factor specifically required for neural cell differentiation. Acts in conjuction with nPTB/PTBP2 by binding directly to its regulated target transcripts and promotes neural-specific exon inclusion in many genes that function in neural cell differentiation. Required to promote the inclusion of neural-specific exon 10 in nPTB/PTBP2, leading to increased expression of neural-specific nPTB/PTBP2. Also promotes the inclusion of exon 16 in DAAM1 in neuron extracts ().
UniProt Protein Details:
NCBI Summary:
SRRM4 promotes alternative splicing and inclusion of neural-specific exons in target mRNAs (Calarco et al., 2009 [PubMed 19737518]).[supplied by OMIM, Oct 2009]
