The SRF Polyclonal Antibody (CAB7731) is a vital tool for researchers studying the Serum Response Factor (SRF), a transcription factor central to the regulation of genes involved in cell growth and differentiation. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By targeting the SRF protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential component for studies in cell biology, developmental biology, and cancer research.SRF is a key player in various cellular processes, including muscle development, neuronal growth, and wound healing.
Dysregulation of SRF has been implicated in diseases such as cancer, cardiovascular disorders, and neurological conditions. By understanding the mechanisms by which SRF influences gene expression, researchers can uncover potential therapeutic targets and develop strategies to manipulate SRF activity for the treatment of various diseases. The SRF Polyclonal Antibody (CAB7731) is a valuable tool for advancing our understanding of SRF function and its impact on human health.
Product Name:
SRF Rabbit Polyclonal Antibody
SKU:
CAB7731
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 321-420 of human SRF (NP_003122.1).
This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6722
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using SRF Rabbit pAb (CAB7731) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
