The SREBF2 Polyclonal Antibody (CAB13049) is a high-quality antibody designed for research involving the Sterol Regulatory Element-Binding Transcription Factor 2 (SREBF2), a key regulator of cholesterol and fatty acid metabolism. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.SREBF2 is a critical transcription factor that controls the expression of genes involved in lipid metabolism, making it a key player in the regulation of cellular lipid levels. Dysregulation of SREBF2 has been linked to various metabolic disorders, including cardiovascular disease, diabetes, and obesity.
Understanding the function of SREBF2 is essential for unraveling the complex pathways involved in lipid homeostasis and metabolic diseases.This antibody specifically binds to SREBF2, allowing for the detection and analysis of this important transcription factor in a variety of cell types. Its high specificity and sensitivity make it an invaluable tool for researchers studying lipid metabolism, metabolic disorders, and related diseases. By exploring the role of SREBF2, researchers can gain valuable insights into the molecular mechanisms underlying metabolic regulation and potentially identify new targets for therapeutic intervention.
Antibody Name:
Anti-SREBP2 Antibody
Antibody SKU:
CAB13049
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IHC IF
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SREBF2 (NP_004590.2).
This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:
Transcriptional activator required for lipid homeostasis. May regulate transcription of the LDL receptor gene as well as the cholesterol and fatty acid synthesis pathways. Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
Western blot analysis of extracts of various cell lines, using SREBP2 antibody (CAB13049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunohistochemistry of paraffin-embedded Human gastric cancer using SREBP2 antibody (CAB13049) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using SREBP2 Rabbit pAb (CAB13049) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using SREBP2 Rabbit pAb (CAB13049) at dilution of 1:100. Blue: DAPI for nuclear staining.
