The SQSTM1 Polyclonal Antibody (CAB22025) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to SQSTM1, this antibody allows for precise detection and analysis of the protein in different cell types, making it ideal for research in the fields of autophagy, inflammation, and cancer biology.SQSTM1, also known as sequestosome 1, plays a key role in cell homeostasis by targeting damaged or unwanted proteins for degradation through autophagy.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SQSTM1 Polyclonal Antibody
SKU:
CAB22025
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Enables K63-linked polyubiquitin modification-dependent protein binding activity; identical protein binding activity; and ionotropic glutamate receptor binding activity. Involved in several processes, including brown fat cell proliferation; energy homeostasis; and positive regulation of long-term synaptic potentiation. Acts upstream of or within negative regulation of transcription by RNA polymerase II. Located in several cellular components, including aggresome; autolysosome; and sperm midpiece. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and sensory organ. Used to study Paget's disease of bone. Human ortholog(s) of this gene implicated in GNE myopathy; Paget's disease of bone; and frontotemporal dementia and/or amyotrophic lateral sclerosis-3. Orthologous to human SQSTM1 (sequestosome 1).
Purification Method
Affinity purification
Gene ID
18412
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using SQSTM1 Rabbit pAb (CAB22025) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of lysates from C2C12 cells, using SQSTM1 Rabbit pAb (CAB22025) at 1:600 dilution. C2C12 cells were treated with Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
